Consequences of a telomerase-related fitness defect and chromosome substitution technology in yeast synIX strains

• DOI: 10.1016/j.xgen.2023.100419
• Publication Date: 2023-11-09T16:50:45Z
• Authors: Laura H. McCulloch; Vijayan Sambasivam; Amanda L. Hughes; Narayana Annaluru; Sivaprakash Ramal...; Viola Fanfani; Evgenii Lobzaev; Leslie A. Mitchell; Jitong Cai; Hua Jiang; John LaCava; Martin S. Taylor; William R. Bishai; Giovanni Stracqua...; Lars M. Steinmetz; Joel S. Bader; Weimin Zhang; Jef D. Boeke; Srinivasan Chandr...; Breeana G. Anderson; Abena Apaw; Pavlo Bohutskyi; Erin Buchanan; Daniel Chang; Melinda Chen; Eric Cooper; Amanda Deliere; Kallie Drakos; Justin Dubin; Christopher Ferna...; Zheyuan Guo; Thomas Harrelson; Dongwon Lee; Jessica McDade; Scott Melamed; Héloise Müller; Adithya Murali; José U. Niño Rivera; Mira Patel; Mary Rodley; Jenna Schwarz; Nirav Shelat; Josh S. Sims; Barrett Steinberg; James Steinhardt; Rishi K. Trivedi; Christopher Von D...; Tianyi Wang; Remus Wong; Yijie Xu; Noah Young; Karen Zeller; Allen Zhang
• Keywords: Human artificial chromosome; Dosage compensation

We describe the complete synthesis, assembly, debugging, and characterization of a synthetic 404,963 bp chromosome,