Neuroprotective effect of selective hypothermic cerebral perfusion in extracorporeal cardiopulmonary resuscitation: A preclinical study

• DOI: 10.1016/j.xjon.2022.07.007
• Publication Date: 2022-08-03T06:40:23Z
• Authors: Kerong Zhai; Mingming Li; Jian Li; Shilin Wei; Zhenzhen Li; Yanchun Zhang; Bingren Gao; Xiangyang Wu; Yongnan Li
• Keywords: Extracorporeal cardiopulmonary resuscitation; Proinflammatory cytokine

Neurologic complications seriously affect the survival rate and quality of life in patients with extracorporeal cardiopulmonary resuscitation (ECPR) undergoing cardiac arrest. This study aimed to repurpose selective hypothermic cerebral perfusion (SHCP) as a novel approach protect brains these patients.Rats were randomly allocated Sham, ECPR, SHCP combined ECPR (CP-ECPR) groups. In group, circulatory was performed at 6 minutes after asphyxial arrest by membrane oxygenation. The vital signs monitored for 3 hours, body brain temperatures maintained normal level. CP-ECPR right carotid artery catheterization serving connected oxygenation device achieve cooling (26-28 °C). Serum markers injury pathomorphologic changes hippocampus evaluated. Three biological replicates further received RNA sequencing Microglia activation inflammatory cytokines tissues serum detected.SHCP rapidly reduced brain-targeted temperature significantly alleviated nerve injury. evident from biomarker levels, lower pathologic scores, more surviving neurons group. Furthermore, differentially expressed genes responses clustered functionally according Kyoto Encyclopedia Genes Genomes pathway analysis. And microglia release proinflammatory mediators.Our preliminary data indicate that may serve potential therapy attenuate via downregulation neuroinflammation ECPR.