High temperature increases centromere-mediated genome elimination frequency and enhances haploid induction in Arabidopsis

• DOI: 10.1016/j.xplc.2022.100507
• Publication Date: 2022-12-20T01:22:03Z
• Authors: Ulkar Ahmadli; Manikandan Kalidass; Lucie Crhak Khaitova; Joerg Fuchs; Maria Cuacos; Dmitri Demidov; Sheng Zuo; Jana Pecinkova; Martin Mascher; Mathieu Ingouff; Stefan Heckmann; Andreas Houben; Karel Riha; Inna Lermontova
• Keywords: Doubled haploidy; Inducer; Wild type

Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, induction via mutation of centromere-specific histone H3 (cenH3) has been shown when mutant outcrossed wild-type, and wild-type genome remains progeny. However, factors that affect are still poorly understood. Here, we report cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as inducer pollinated by wild-type. We discovered short-term temperature stress knl2 increased efficiency 10-fold. also demonstrated point CENPC-k motif KNL2 sufficient generate haploid-inducing lines, suggesting crops identified naturally occurring or chemically induced population, avoiding generic modification (GM) approach at any stage. Furthermore, cenh3-4 functioned response heat stress, even though it did not induce haploids under standard conditions. Thus, new target gene for generation haploid-inducer showed exposure centromeric protein mutants high strongly increases their efficiency.