Centromere repositioning and shifts in wheat evolution

• DOI: 10.1016/j.xplc.2023.100556
• Publication Date: 2023-02-03T18:18:52Z
• Authors: Jing Zhao; Yilin Xie; Chuizheng Kong; Zefu Lu; Haiyan Jia; Zhengqiang Ma; Yijing Zhang; Dangqun Cui; Zhengang Ru; Yuquan Wang; Rudi Appels; Jizeng Jia; Xueyong Zhang
• Keywords: Chromatin immunoprecipitation

The centromere is the region of a chromosome that directs its separation and plays an important role in cell division reproduction organisms. Elucidating dynamics centromeres alternative strategy for exploring evolution wheat. Here, we comprehensively analyzed from de novo-assembled common wheat cultivar Aikang58 (AK58), Chinese Spring (CS), all sequenced diploid tetraploid ancestors by chromatin immunoprecipitation sequencing, whole-genome bisulfite RNA assay transposase-accessible using comparative genomics. We found centromere-associated sequences were concentrated during tetraploidization hexaploidization. Centromeric repeats (CRWs) have undergone expansion evolution, with strong interweaving between A B subgenomes post tetraploidization. CENH3 prefers to bind younger CRWs, as directly supported immunocolocalization on two chromosomes (1A 2A) wild emmer dicentromeric regions, only one which bound CENH3. In comparison AK58 CS, obvious repositioning was detected 1B, 3D, 4D. active showed unique combination lower CG but higher CHH CHG methylation levels. also centromeric more open than pericentromeric chromatin, levels gene expression density. Frequent introgression hexaploid had influence position same chromosome. This study genetically epigenetically determined.