CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models

• DOI: 10.1016/j.xpro.2021.101038
• Publication Date: 2022-01-07T13:58:04Z
• Authors: Maike Effern; Nicole Glodde; Emma Bawden; Jana Liebing; Daniel Hinze; Thomas Tüting; Thomas Gebhardt; Michael Hölzel
• Keywords: Adoptive Cell Transfer; Cell therapy

This protocol details the procedure for CRISPR-assisted insertion of epitopes (CRISPitope), a flexible approach generating tumor cells expressing model CD8+ T cell fused to endogenously encoded gene products choice. CRISPitope-engineered can be recognized by receptor-transgenic (TCRtg) that are widely used in immunology research. Using mice inoculated with cells, researchers investigate how choice target antigen immunotherapies influences treatment efficacy and resistance mechanisms. For complete on use execution this protocol, please refer Effern et al. (2020).