Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages

• DOI: 10.1016/j.xpro.2021.101080
• Publication Date: 2022-01-08T00:11:36Z
• Authors: Zhenzhen Yan; Hansen Liu; Chengjiang Gao
• Keywords: Viral infection

Upon viral infection, several proteins in the innate signaling pathway form aggregates, which turn promote activation of antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peritoneal macrophages, and show how detect aggregation TBK1 upon infection. The protocol is adaptable for other viruses. For complete details on execution profile, please refer Yan et al. (2021).