Epithelial cell spreading assay on E-cadherin-coated glass or PDMS substrates for microscopy-based analysis of cadherin adhesions

• DOI: 10.1016/j.xpro.2023.102626
• Publication Date: 2023-10-03T16:33:46Z
• Authors: Lilian Schimmel; Ivar Noordstra
• Keywords: Adherens junction; Polydimethylsiloxane

Adherens junctions (AJs) are multi-protein adhesion structures that couple contractile actomyosin networks of epithelial cells within a tissue. Here, we present an cell spreading assay on E-cadherin-coated glass or polydimethylsiloxane (PDMS) substrates for detailed microscopy-based analysis cadherin adhesions. We describe steps preparation coverslips and PDMS gels, E-cadherin coating, spreading. Epithelial can be seeded surfaces, thereby mimicking AJ formation in X-Y dimension, making it suitable microscopy analysis. For complete details the use execution this protocol, please refer to Noordstra et al. (2023).1