HNRNPU promotes the progression of hepatocellular carcinoma by enhancing CDK2 transcription

• DOI: 10.1016/j.yexcr.2021.112898
• Publication Date: 2021-10-29T08:01:11Z
• Authors: Yi Liang; Yao Fan; Yu Liu; Hui Fan
• Keywords: 0301 basic medicine; Mice, Inbred BALB C; Carcinoma, Hepatocellular; Transcription, Genetic; Cyclin-Dependent Kinase 2; Liver Neoplasms; Down-Regulation; Mice, Nude; Apoptosis; Hep G2 Cells; Heterogeneous-Nuclear Ribonucleoprotein U; Cell Line; Up-Regulation; Gene Expression Regulation, Neoplastic; Mice; 03 medical and health sciences; HEK293 Cells; Cell Line, Tumor; Animals; Humans; Female; Cell Proliferation

The nuclear matrix-associated protein Heterogeneous Nuclear Ribonucleoprotein U (HNRNPU), also known as SAF-A, is to maintain active chromatin structure in mouse hepatocytes. However, the functional roles and molecular mechanisms of HNRNPU development hepatocellular carcinoma (HCC) remain largely unknown. Herein, we found that was upregulated HCC, proliferation HCC cells inhibited vitro vivo upon knockdown. Moreover, upregulation correlated with poor prognosis HCC. Mechanistically, bound CDK2 gene locus, a key factor cell cycle regulation, where it enriched H3K27 acetylation (H3K27ac), H3K9 (H3K9ac), H3K4 mono-methylation (H3K4me1). Furthermore, knockdown reduced levels H3K27ac H3K9ac at binding site, tri-methylation (H3K27me3) were increased, eventually leading downregulation CDK2. Collectively, our results provide new mechanism whereby promotes by enhancing transcription