Genome-wide circular RNA (circRNA) and mRNA profiling identify a circMET-miR-410-3p regulatory motif for cell growth in colorectal cancer

• DOI: 10.1016/j.ygeno.2021.11.038
• Publication Date: 2021-12-18T03:39:38Z
• Authors: Yining Liu; Lei Chen; Tianqi Liu; Xiangqian Su; Lin Peng; Jiangbo Chen; Fei Tan; Pu Xing; Zaozao Wang; Jiabo Di; Beihai Jiang; Hong Qu
• Keywords: Competing Endogenous RNA; Circular RNA

Circular RNA (circRNA) is a non-coding molecule that lacks polyadenylated tails and highly stable, abundant, conserved in human cells. CircRNAs can serve as competing endogenous (ceRNA) to sponge microRNAs (miRNA) block their effects on target mRNA expression. also have possible relevance cancer therefore may be considered ideal biomarkers for monitoring progression. Of the about 300,000 predicted circRNAs, only few validated biological functions related cancer. To better understand ceRNA role of circRNAs colorectal (CRC), we performed genome-wide circRNA-based RNA-sequencing (RNA-Seq) nine CRC tumor samples paired histologically normal adjacent tissue samples. By profiling expression same patients, further explored correlation between mRNAs generated from parental gene. Focusing concordant differential mRNAs, substantially reduced regulatory noise. In total, identified 694 circRNA-mRNA pairs were consistently up or downregulated tissues. These are 182 protein-coding genes associated with hormone responses chemotaxis. these genes, 43 downstream targets three miRNAs (miR-410-3p, miR-135a, miR-30a). Interestingly, mutated another cohort eight independent studies, which significant patient survival time. miR-410-3p its oncogene MET, experimentally motif circMET. Notably, circMET upregulated cell lines could promote proliferation growth. confirming relationship circMET, propose new mechanism observed sustained activation MET CRC. conclusion, our work identifies novel provides potential diagnostic biomarker