Phylotranscriptomic relationships of the Oophila clade of green algae associated to amphibian egg masses

• DOI: 10.1016/j.ympev.2024.108165
• Publication Date: 2024-08-07T00:02:53Z
• Authors: Miguel Vences; Maria Sachs; Iker Irisarri; Fabian Bartels; Pontus F. Eriksson; Sven Künzel; Atsushi Kurabayashi; Ane T. Laugen; Zachary T. Vegso; Cory D. Bishop; Ryan Kerney; Hartmut Arndt
• Keywords: Subclade

Green algae usually assigned to the genus Oophila are known colonize egg capsules of amphibian masses across Nearctic and Palearctic regions. We study phylogenetic relationships these using a phylotranscriptomic data set 76 protein-coding single-copy nuclear genes. Our includes novel RNAseq for six amphibian-associated five free-living green algae, draft genomes two latter. Within clade (nested within Moewusinia), we find samples from European frogs (Rana dalmatina R. temporaria) closely related those North American frog aurora (Oophila subclade III). An isolate sylvatica (subclade IV) appears be sister Japanese salamander Hynobius nigrescens J1), I Ambystoma maculatum all other lineages in clade. Two (Chlamydomonas nasuta Cd. pseudogloeogama) nested clade, strain type species Chlorococcum (Cc. infusionum) is this assemblage. tree suggests that recognition different ("clade B" earlier studies) warranted, calls comprehensive taxonomic revision Moewusinia.