MCF-7 breast cancer cells express normal forms of all vasopressin receptors plus an abnormal V2R☆

• DOI: 10.1016/s0196-9781(99)00070-4
• Publication Date: 2002-07-25T23:20:22Z
• Authors: William G. North; Michael J. Fay; Jinlin Du
• Keywords: 0301 basic medicine; Receptors, Vasopressin; Base Sequence; Reverse Transcriptase Polymerase Chain Reaction; Molecular Sequence Data; Breast Neoplasms; DNA, Neoplasm; 3. Good health; 03 medical and health sciences; Tumor Cells, Cultured; Humans; Female; RNA, Messenger; RNA, Neoplasm; Cloning, Molecular; DNA Primers

We have previously provided evidence that an autocrine loop involving vasopressin is present in perhaps all breast cancers. This study now shows MCF-7 cancer cells express mRNAs for currently recognized receptor subtypes (V1a, V1b, and V2). Cloning DNA sequencing over the entire open reading frame of each mRNA revealed normal sequences representing were present. However, addition, abnormal V2 receptor, expected to give rise a truncated 'diabetic' protein, was also expressed. Western analysis three gave proteins sizes compatible with them being functional receptors. The proteins.