Effect of dietary fibre of barley variety ‘Rihane’ on azoxymethane-induced aberrant crypt foci development and on colonic microbiota diversity in rats

• DOI: 10.1017/s0007114512000219
• Publication Date: 2012-03-14T11:00:07Z
• Authors: Lamia Lahouar; Philippe Pochart; Hichem Ben Salem; Mouledi El Felah; Moncef Mokni; Fabien Magne; Irène Mangin; Antonia Suau; Ester Pereira; Mohamed Hammami; Lotfi Achour
• Keywords: Aberrant crypt foci; Dietary fibre

Many epidemiological and experimental studies have suggested an important role for dietary fibre (DF) of cereals in the prevention colon cancer. The objective present study was to explain effects DF barley Rihane (BR) on azoxymethane (AOM)-induced aberrant crypt foci (ACF) colonic bacterial diversity rats. Following acclimatisation period, rats were divided into four groups fed a control (C) diet or containing 30 % BR. content twice that C (total 8·69 15·24 BR diet). At 7 8 weeks age, received two successive subcutaneous injections AOM at 20 mg/kg body weight. 12 after first injection, ten animals from each group killed. decreased pH (P < 0·05) compared with diet. total number ACF observed considerably BR/AOM C/AOM 0·05). Comparison similarity coefficients showed variability microbiota species between different groups. In addition, we inter-individual within same group. This affected by AOM. results show bifidobacteria numbers lower those However, enterobacteria increased reduced incidence AOM-induced biodiversity.