Soyabean glycinin depresses intestinal growth and function in juvenile Jian carp (Cyprinus carpiovar Jian): protective effects of glutamine

• DOI: 10.1017/s0007114515003219
• Publication Date: 2015-09-09T05:44:25Z
• Authors: Wei-Dan Jiang; Kai Hu; Jin-Xiu Zhang; Yang Liu; Jun Jiang; Pei Wu; Juan Zhao; Sheng-Yao Kuang; Ling Tang; Wu-Neng Tang; Yong-An Zhang; Xiao-Qiu Zhou; Lin Feng
• Keywords: Inflammation; 2. Zero hunger; Glutathione Peroxidase; Carps; Glutamine; Proteins; Apoptosis; Globulins; 04 agricultural and veterinary sciences; Catalase; Antioxidants; Diet; Intestines; Fish Diseases; Oxidative Stress; Glutathione Reductase; Soybean Proteins; Animals; 0401 agriculture, forestry, and fisheries; Lipid Peroxidation; RNA, Messenger; Oxidation-Reduction; Glutathione Transferase

AbstractThis study investigated the effects of glycinin on the growth, intestinal oxidative status, tight junction components, cytokines and apoptosis signalling factors of fish. The results showed that an 80 g/kg diet of glycinin exposure for 42 d caused poor growth performance and depressed intestinal growth and function of juvenile Jian carp (Cyprinus carpiovar. Jian). Meanwhile, dietary glycinin exposure induced increases in lipid peroxidation and protein oxidation; it caused reductions in superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities; and it increasedMnSOD, CuZnSOD, GPx1b and GPx4amRNA levels, suggesting an adaptive mechanism against stress in the intestines of fish. However, dietary glycinin exposure decreased both the activity and mRNA levels of nine isoforms of glutathione-S-transferase (GST) (α,μ,π,ρ,θ,κ,mGST1,mGST2andmGST3), indicating toxicity to this enzyme activity and corresponding isoform gene expressions. In addition, glycinin exposure caused partial disruption of intestinal cell–cell tight junction components, disturbances of cytokines and induced apoptosis signalling in the distal intestines>mid intestines>proximal intestines of fish. Glycinin exposure also disturbed the mRNA levels of intestinal-related signalling factorsNrf2,Keap1a,Keap1b, eleven isoforms of protein kinase C and target of rapamycin/4E-BP. Interestingly, glutamine was observed to partially block those negative influences. In conclusion, this study indicates that dietary glycinin exposure causes intestinal oxidative damage and disruption of intestinal physical barriers and functions and reduces fish growth, but glutamine can reverse those negative effects in fish. This study provides some information on the mechanism of glycinin-induced negative effects.