Evaluation ofBt-toxin uptake by the non-target herbivore,Myzus persicae(Hemiptera: Aphididae), feeding on transgenic oilseed rape

• DOI: 10.1017/s0007485307004920
• Publication Date: 2007-04-05T14:22:24Z
• Authors: G. Burgio; A. Lanzoni; G. Accinelli; G. Dinelli; A. Bonetti; I. Marotti; F. Ramilli
• Keywords: Myzus persicae; Cry1Ac; Bacillus thuringiensis; Biosafety

Abstract As consequence of the concern about biosafety genetically modified plants, biological and ecological studies are considered crucial for environmental risk assessment. Laboratory experiments were carried out in order to evaluate transfer Cry1Ac Bt -toxin from a transgenic -oilseed rape non-target pest, Myzus persicae Sulzer. protein levels plants aphids determined using double sandwich enzyme-linked immunosorbent assay. Phloem sap ( +) −) oilseed was collected leaves standard method extraction an EDTA buffer. present phloem sap, with mean concentration 2.7±1.46 ppb, corresponding 24-fold lower level than leaves. Toxin also detected aphid samples, positive samples 2.0±0.8 ppb. The evidence that remains herbivores, this case aphid, could be useful clarify functional aspects linked possible consequences -crops on food chains involving herbivore–natural enemy trophic systems. Further needed improve knowledge GM-oilseed their consequence.