Transcriptional and physiological changes of alfalfa in response to aluminium stress

• DOI: 10.1017/s0021859611000256
• Publication Date: 2011-03-17T09:20:34Z
• Authors: Q. CHEN; X. D. ZHANG; S. S. WANG; Q. F. WANG; G. Q. WANG; H. J. NIAN; K. Z. LI; Y. X. YU; L. M. CHEN
• Keywords: Suppression subtractive hybridization; Malondialdehyde

SUMMARY Medicago sativa is an excellent pasture legume, but it very sensitive to aluminium (Al) toxicity. To better understand the mechanism of M. sensitivity Al, a forward suppression subtractive hybridization (SSH) cDNA library for Al-sensitive cultivar, L. cv. Yumu No. 1 (YM1), under 5 μ m Al stress over 24 h period was constructed analyse changes in its gene expression response stress. Sequence analysis SSH generated 291 high-quantity sequence tags (ESTs). Of these, 229 were known as functional ESTs, 137 which have already been reported genes, whereas other 92 potentially novel Al-associated genes. The up-regulation resistance-associated genes encoding transcription factor proton rhizotoxicity ( STOP1 ) and malate transporter MsALMT1 (Al-activated transporter) well antioxidant enzymes observed. Reverse polymerase chain reaction validated reliability data confirmed up-regulated physiological indicated that hydrogen peroxide (H 2 O malondialdehyde levels elevated rapidly stress, suggesting severe oxidative occurred YM1 roots. antioxidant-related might be important protective induced by Al-induced exudation increased drastically during early after treatment, due function MsALMT . However, from roots declined quickly subsequent period, gradual decrease content simultaneously observed This result agreement with observation organic acid metabolism-associated such phosphoenolpyruvate carboxylase, citrate synthase dehydrogenase not present library. major reason Al.