Migration ofFasciola hepaticanewly excysted juveniles is inhibited by high-mannose and oligomannose-typeN-glycan-binding lectins

• DOI: 10.1017/s003118201700124x
• Publication Date: 2017-07-10T09:24:04Z
• Authors: ANDRES GARCIA-CAMPOS; ALAN W. BAIRD; GRACE MULCAHY
• Keywords: Viral tegument

Fasciola hepatica has both zoonotic importance and high economic impact in livestock worldwide. After ingestion by the definitive host, Newly Excysted Juveniles (NEJ) penetrate intestine before reaching peritoneal cavity. The role of some NEJ-derived proteins invasion been documented, but NEJ glycans or lectin-binding receptors during initial infection gut is still unknown. To address these questions, migration through rat was recorded at 30 min intervals up to 150 two ex vivo methods. Firstly, jejunal sheets were challenged with incubated biotinylated lectins. Secondly, untreated distal jejunum pre-treated Both Concanavalin A (ConA) Galanthus nivalis (GNL), which recognize mannose-type N-glycans, significantly inhibited across jejunum. Most lectins bound tegument oral sucker NEJ, only ConA GNL maintained this interaction over min. None examined reduced when pre-incubated sheets, suggesting that host might not be essential for binding/recognition NEJ. Agents capable blocking N-glycans on may have potential disrupting infection.