Generation and characterization of U937-TR: a platform cell line for inducible gene expression in human macrophages

• DOI: 10.1017/s0031182020001110
• Publication Date: 2020-07-27T07:46:50Z
• Authors: Cristian Camilo G...; Carlos Arturo Cla...
• Keywords: U937 cell

Abstract Monocytes and macrophages are involved in a wide range of biological processes parasitic diseases. The characterization the molecular mechanisms governing such usually requires precise control expression genes interest. We implemented tetracycline-controlled gene system U937 cell line, one most used vitro models for research human monocytes macrophages. Here we characterized U937-derived lines terms phenotypic (morphology marker expression) functional (capacity phagocytosis Leishmania parasite hosting) changes induced by phorbol-12-myristate-13-acetate (PMA). Finally, provide evidence tetracycline-inducible reversible Lamin-A silencing PMA-differentiated cells.