Molecular identification of the first local dengue fever outbreak in Shenzhen city, China: a potential imported vertical transmission from Southeast Asia?

• DOI: 10.1017/s0950268813000897
• Publication Date: 2013-04-15T16:48:11Z
• Authors: F. YANG; G. Z. GUO; J. Q. CHEN; H. W. MA; T. LIU; D. N. HUANG; C. H. YAO; R. L. ZHANG; C. F. XUE; L. ZHANG
• Keywords: Molecular Epidemiology

SUMMARY A suspected dengue fever outbreak occurred in 2010 at a solitary construction site Shenzhen city, China. To investigate this epidemic, we used serological, molecular biological, and bioinformatics techniques. Of nine serum samples from patients, detected seven positive for virus (DENV) antibodies, eight DENV-1 RNA, three containing live viruses. The isolated virus, SZ1029 strain, was sequenced confirmed as DENV-1, showing the highest E-gene homology to D1/Malaysia/36000/05 SG(EHI)DED142808 strains recently reported Southeast Asia. Further phylogenetic tree analysis their close relationship. At epidemic site, also 14 asymptomatic co-workers (out of 291) DENV antibody, DENV-1-positive mosquitoes. Thus, concluded that caused first local Shenzhen. Because no imported case identified, fingerprints strain suggest may be due vertical transmission