FGF18 modulates CTGF mRNA expression in cumulus–oocyte complexes and early bovine embryos: preliminary data

• DOI: 10.1017/s0967199421000599
• Publication Date: 2021-08-18T11:03:49Z
• Authors: Elisabeth Schmidt...; Carolina Amaral; Marcos Barreta; Alfredo Antoniazzi; Leonardo Guedes d...; Rogério Ferreira; Fernando Mesquita; Valério Marques P...; Paulo Bayard Gonç...
• Keywords: 0301 basic medicine; Cumulus Cells; Embryonic Development; In Vitro Oocyte Maturation Techniques; Fibroblast Growth Factors; 03 medical and health sciences; Blastocyst; Pregnancy; Oocytes; Animals; Cattle; Female; RNA, Messenger; Preliminary Data

The Hippo pathway is involved in the proliferation of intrafollicular cells and early embryonic development, mainly because effectors this are key transcription regulators genes such as CTGF CYR61, which cell proliferation. Recent studies by our group found that fibroblast growth factor 18 (FGF18) present fallopian tube during leading to hypothesis FGF18 may have a role development. Therefore, aim following study was determine whether modulates expression target genes, oocyte maturation Three experiments were carried out, with vitro (IVM) cumulus-oocyte complexes (COCs) embryo culture. In experiment one, (100 ng/ml) induced an increase (P < 0.05) gene at 12 h post-exposure. two, reduction 3 third experiment, day 7 embryos exposed IVM expressed greater mRNA abundance, whereas exposure culture did not alter comparison untreated controls. preliminary data presented here show critical periods nuclear maturation, cumulus expansion development cattle.