Fluoroimmunoassay for Antigen Based on Fluorescence Quenching Signal of Gold Nanoparticles

• DOI: 10.1021/ac051323m
• Publication Date: 2006-02-14T05:35:55Z
• Authors: Limei Ao; Feng Gao; Bifeng Pan; Rong He; Daxiang Cui
• Keywords: SIGNAL (programming language)

A unique, sensitive, and highly specific fluoroimmunoassay system for antigen detection using gold magnetic nanoparticles has been developed. The assay is based on the fluorescence quenching of fluorescein isothiocyanate caused by coated with monoclonal antibody. To demonstrate its analytical capabilities, were anti-alpha-fetoprotein polyclonal antibodies, which specifically bound alpha-fetoprotein. Gold antibodies could sandwich alpha-fetoprotein captured nanoparticle probes. sandwich-type immunocomplex was formed surface be separated a field. supernatant liquid, contained unbound probes, used to quench fluorescence, intensity at 516 nm proportional concentration. result showed that limit 0.17 nM. This new can extended detect target molecules matched broad potential applications in immunoassay disease diagnosis.