Use of Real-Time, Label-Free Analysis in Revealing Low-Affinity Binding to Blood Group Antigens by Helicobacter pylori

• DOI: 10.1021/ac201260c
• Publication Date: 2011-07-01T21:34:52Z
• Authors: Y. Y. Fei; A. Schmidt; G. Bylund; D. X. Johansson; S. Henriksson; C. Lebrilla; J. V. Solnick; T. Borén; X. D. Zhu
• Keywords: Glycoconjugate

Infectious diseases are often initiated by microbial adherence that is mediated the binding of attachment molecules, termed adhesins, to cell surface receptors on host cells. We present an experimental system, oblique-incidence reflectivity difference (OI-RD) microscopy, which allows detection novel, low-affinity mechanisms may be essential for infectious processes. OI-RD microscopy was used analyze direct oncopathogen, Helicobacter pylori ( H. ) immobilized glycoconjugates in real time with no need labeling tags. The results suggest presence additional Lewis b blood group antigen (Le(b)) adhesins have not been detected previously. also confirmed high-affinity outer-membrane protein BabA Le(b). method broadly applicable real-time characterization intact and offers new strategies elucidate molecular interactions agents human