Development of a Long-Lived Luminescence Probe for Visualizing β-Galactosidase in Ovarian Carcinoma Cells

• DOI: 10.1021/acs.analchem.7b03114
• Publication Date: 2017-10-03T08:29:43Z
• Authors: Wanhe Wang; Kasipandi Vellaisamy; Guodong Li; Chun Wu; Chung-Nga Ko; Chung-Hang Leung; Dik-Lung Ma
• Keywords: Luminescent Measurements

β-Galactosidase (β-gal) is an important biomarker for ovarian cancers. In this work, we designed and synthesized a novel iridium(III)-based probe 1 discriminating carcinoma cell lines from normal lines. The could detect β-gal even in the presence of highly autofluorescent background. also showed good linear response to between 0 30 U/mL, with detection limit 0.51 U/mL. Importantly, complex selectively "light up" cells, while exhibiting negligible luminescence cells. Overall, be potentially used as useful detecting expression context cancer diagnostics.