Evidence of an Unusual Poly(A) RNA Signature Detected by High-Throughput Chemical Mapping

• DOI: 10.1021/acs.biochem.0c00215
• Publication Date: 2020-05-15T23:44:26Z
• Authors: Roger Wellington-...; Eli Fisker; Mathew Zada; Michelle Wiley; Jill Townley; Eterna Players
• Keywords: Primer extension; Signature (topology); Substitution (logic); Sequence (biology)

Homopolymeric adenosine RNA plays numerous roles in both cells and noncellular genetic material. We report herein an unusual poly(A) signature chemical mapping data generated by the Eterna Massive Open Laboratory. Poly(A) sequences of length seven or more show unexpected results selective 2′-hydroxyl acylation read out primer extension (SHAPE) dimethyl sulfate (DMS) probing. This first appears grows to its maximum strength at ∼10. In a long sequence, substitution single A any other nucleotide disrupts signature, but only for 6 so nucleotides on 5′ side substitution.