A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA

• DOI: 10.1021/acs.biochem.2c00270
• Publication Date: 2022-07-25T14:22:01Z
• Authors: Natalie Falco; Chely M. Garfio; Leslie Spitalny; Robert C. Spitale
• Keywords: Transcription; Inosine

Herein, we detail a novel reverse-transcription (RT) assay to directly detect chemical adducts on RNA. We optimize fluorescence quenching RT polymerization and employ our approach N1-alkylation of inosine, an important post-transcriptional modification, using phenylacrylamide as model compound. anticipate can be expanded identify reagents that form with RNA further explored understand the relationship between processivity natural modifications in