The WYL Domain of the PIF1 Helicase from the Thermophilic Bacterium Thermotoga elfii is an Accessory Single-Stranded DNA Binding Module

• DOI: 10.1021/acs.biochem.7b01233
• Publication Date: 2018-01-17T20:45:55Z
• Authors: Nicholas M. Andis; Christopher W. Sa...; Ashna Alladin; Matthew L. Bochman
• Keywords: Adenosine Triphosphatases; 0301 basic medicine; 0303 health sciences; Binding Sites; DNA Helicases; DNA, Single-Stranded; Gram-Negative Anaerobic Straight, Curved, and Helical Rods; 03 medical and health sciences; Bacterial Proteins; Protein Domains; Amino Acid Sequence; Sequence Alignment; Protein Binding

ABSTRACTPIF1 family helicases are conserved from bacteria to man. With the exception of the well-studied yeast PIF1 helicases (e.g., ScPif1 and ScRrm3), however, very little is known about how these enzymes help maintain genome stability. Indeed, we lack a basic understanding of the protein domains found N- and C-terminal to the characteristic central PIF1 helicase domain in these proteins. Here, using chimeric constructs, we show that the ScPif1 and ScRrm3 helicase domains are interchangeable and that the N-terminus of ScRrm3 is important for its functionin vivo. This suggests that PIF1 family helicases evolved functional modules fused to a generic motor domain. To investigate this hypothesis, we characterized the biochemical activities of the PIF1 helicase from the thermophilic bacteriumThermotoga elfii(TePif1), which contains a C-terminal WYL domain of unknown function. Like helicases from other thermophiles, recombinant TePif1 was easily prepared, thermostablein vitro, and displayed activities similar to its eukaryotic homologs. We also found that the WYL domain was necessary for high-affinity single-stranded DNA (ssDNA) binding and affected both ATPase and helicase activities. Deleting the WYL domain from TePif1 or mutating conserved residues in the predicted ssDNA binding site uncoupled ATPase activity and DNA unwinding, leading to higher rates of ATP hydrolysis but less efficient DNA helicase activity. Our findings suggest that the domains of unknown function found in eukaryotic PIF1 helicases may also confer functional specificity and additional activities to these enzymes, which should be investigated in future work.