Cellulose Mesh with Charged Nanocellulose Coatings as a Promising Carrier of Skin and Stem Cells for Regenerative Applications

• DOI: 10.1021/acs.biomac.0c01097
• Publication Date: 2020-11-02T20:15:25Z
• Authors: Julia Pajorova; Anne Skogberg; Daniel Hadraba; Antonin Broz; Martina Travnickova; Marketa Zikmundova; Mari Honkanen; Markus Hannula; Panu Lahtinen; Maria Tomkova; Lucie Bacakova; Pasi Kallio
• Keywords: Nanocellulose

Engineering artificial skin constructs is an ongoing challenge. An ideal material for hosting cells still to be discovered. A promising candidate low-cost cellulose, which commonly fabricated in the form of a mesh and applied as wound dressing. Unfortunately, structure topography current cellulose meshes are not optimal cell growth. To enhance surface physicochemical properties commercially available mesh, we coated with wood-derived nanofibrils (CNFs). Three different types coatings proposed this study carrier: positively charged cationic (cCNFs), negatively anionic (aCNFs), combination these two materials (c+aCNFs). These carriers were seeded normal human dermal fibroblasts (NHDFs) or adipose-derived stem (ADSCs) investigate adhesion, spreading, morphology, proliferation. The aCNF coating significantly improved proliferation both types. cCNF enhanced adhesion ADSCs only. number NHDFs was similar on noncoated pristine mesh. However, three-dimensional (3D) promoted survival. c+aCNF construct proved combine benefits from CNFs, means that carrier further application tissue engineering.