Quaternized Chitosan/Heparin Polyelectrolyte Multilayer Films for Protein Delivery

• DOI: 10.1021/acs.biomac.2c00926
• Publication Date: 2022-10-27T04:16:39Z
• Authors: Tomasz Urbaniak; Gabriela S. Garcí...; Alexander Zhigunov; Sviatoslav Hladysh; Edyta Adrian; Volodymyr Lobaz; Tereza Krunclová; Olga Janoušková; Ognen Pop-Georgie...; Dana Kubies
• Keywords: Biomaterial; Tannic acid; Biopolymer

Layer-by-layer (LbL) polyelectrolyte coatings are intensively studied as reservoirs of bioactive proteins for modulating interactions between biomaterial surfaces and cells. Mild conditions the incorporation growth factors into delivery systems required to maintain protein bioactivity. Here, we present LbL films composed water-soluble N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride (HTCC), heparin (Hep), tannic acid (TA) fabricated under physiological with ability release heparin-binding proteins. Surface plasmon resonance analysis showed that formed on an anchoring HTCC/TA bilayer, TA serving a physical crosslinker, were more stable during their assembly, leading increased film thickness release. X-ray reflectivity measurements confirmed intermixing deposited layers. Protein also when integral part Hep layers rather than individual The 4-week pattern depended type; VEGF, CXCL12, TGF-β1 exhibited typical high initial release, whereas FGF-2 was sustainably released over 4 weeks. Notably, nontoxic, retained bioactivity, demonstrated by intensive chemotaxis T-lymphocytes in response CXCL12. Therefore, proposed promising coating candidates stimulating cellular responses.