Deciphering the Inhibition of Ethane on Anaerobic Ammonium Oxidation

• DOI: 10.1021/acs.est.2c01527
• Publication Date: 2022-08-02T17:56:36Z
• Authors: Xin Tan; Wen-Bo Nie; Guo-Jun Xie; Cheng-Cheng Dang; Xiao-Wei Wang; Defeng Xing; Bing-Feng Liu; Jie Ding; Nanqi Ren
• Keywords: Anammox; Ammonia monooxygenase

Anaerobic ammonium oxidation (anammox) and nitrification, two common biological pathways, are critical for the microbial nitrogen cycle. Short chain alkanes (C2-C8) have been well-known as inhibitors nitrification through interaction with ammonia monooxygenase, while whether these affect anammox is an open question. Here, this work demonstrated significant inhibition of ethane on revealed inhibitory mechanism. The acute was concentration-dependent reversible; 0.86 mM dissolved caused 50% (IC50), 1.72 almost completely inhibited anammox. After long-term exposure to 0.09 30 days, (nitrite) removal rate dropped from 202 (267) mg N L-1 d-1 1 (1) d-1, abundance bacteria decreased 61.9% 9.5%. intercellular concentration after exposure, metatranscriptome analysis showed upregulation genes transport bacteria. Thus, could suppress uptake resulting in activities. As second most prevalent alkane methane various anoxic environments, may important effect cycle driven by that should be investigated future research.