Role of Secondary Organic Matter on Soot Particle Toxicity in Reconstituted Human Bronchial Epithelia Exposed at the Air–Liquid Interface

• DOI: 10.1021/acs.est.2c03692
• Publication Date: 2022-11-23T12:02:31Z
• Authors: Zaira Leni; Michaela N. Ess; Alejandro Keller; James D. Allan; Heidi Hellén; Karri Saarnio; Katie R. Williams; Andrew S. Brown; Matthias Salathe; Nathalie Baumlin; Konstantina Vasil...; Marianne Geiser
• Keywords: Particle (ecology)

Secondary organic matter (SOM) formed from gaseous precursors constitutes a major mass fraction of fine particulate matter. However, there is only limited evidence on its toxicological impact. In this study, air–liquid interface cultures human bronchial epithelia were exposed to different series fresh and aged soot particles generated by miniCAST burner combined with micro smog chamber (MSC). Soot cores geometric mean mobility diameters 30 90 nm coated increasing amounts SOM, the photo-oxidation mesitylene ozonolysis α-pinene. At 24 h after exposure, release lactate dehydrogenase (LDH), indicating cell membrane damage, was measured proteome analysis, i.e. 102 cytokines chemokines assess inflammatory response, performed. The data indicate that presence SOM coating bioavailability play an important role in cytotoxicity. particular, LDH increased mass/total particle ratio, but when had condensed outer surface cores. Proteome analysis provided further for substantial interference essential properties respiratory epithelium as barrier well affecting remodeling activity.