Microbial Niche Differentiation during Nitrite-Dependent Anaerobic Methane Oxidation

• DOI: 10.1021/acs.est.2c08094
• Publication Date: 2023-04-12T02:54:56Z
• Authors: Wen-Bo Nie; Guo-Jun Xie; Xin Tan; Jie Ding; Yang Lu; Yi Chen; Chun Yang; Qiang He; Bing-Feng Liu; Defeng Xing; Nanqi Ren
• Keywords: Kinetics; Candidatus Methylomirabilis oxyfera; Bacteria; Nitrogen Dioxide; kinetic analysis; Anaerobiosis; nitrite-dependent anaerobic methane oxidation; Candidatus Methylomirabilis sinica; Methane; Oxidation-Reduction; genome comparison; Nitrites; niche differentiation

Nitrite-dependent anaerobic methane oxidation (n-DAMO) has been demonstrated to play important roles in the global and nitrogen cycle. However, despite diverse n-DAMO bacteria widely detected environments, little is known about their physiology for microbial niche differentiation. Here, we show differentiation of through long-term reactor operations combining genome-centered omics kinetic analysis. With same inoculum dominated by both species "Candidatus Methylomirabilis oxyfera" sinica", bacterial population was shifted "Ca. M. a fed with low-strength nitrite, but sinica" high-strength nitrite. Metatranscriptomic analysis showed that harbored more complete function cell chemotaxis, flagellar assembly, two-component system better uptake while had active ion transport stress response system, redundant nitrite reduction mitigate inhibition. Importantly, half-saturation constant (0.057 mM vs 0.334 NO2-) inhibition thresholds (0.932 2.450 respectively, were highly consistent genomic results. Integrating these findings biochemical characteristics, especially kinetics affinity determine bacteria.