Construction of a Synthetic Microbial Community for Enzymatic Pretreatment of Wheat Straw for Biogas Production via Anaerobic Digestion

• DOI: 10.1021/acs.est.4c02789
• Publication Date: 2024-05-15T20:05:13Z
• Authors: Jinmeng Chen; Yafan Cai; Zhi Wang; Songtao Wang; Jia Li; Chuan Song; Wei Zhuang; Dong Liu; Shilei Wang; Andong Song; Jingliang Xu; Hanjie Ying
• Keywords: Biogas; Biogas Production; Digestion; Enzymatic Hydrolysis; Microbial consortium

Biological pretreatment is a viable method for enhancing biogas production from straw crops, with the improvement in lignocellulose degradation efficiency being crucial factor this process. Herein, metagenomic approach was used to screen core microorganisms (Bacillus subtilis, Acinetobacter johnsonii, Trichoderma viride, and Aspergillus niger) possessing lignocellulose-degrading abilities among samples three environments: pile retting wheat (WS), WS returned soil, forest soil. Subsequently, synthetic microbial communities were constructed fermentation–enzyme production. The crude enzyme solution obtained pretreat compared two commercial enzymes. community enzyme-producing (SMCEP) yielded highest enzymatic digestion efficacy WS, yielding cellulose, hemicellulose, lignin rates of 39.85, 36.99, 19.21%, respectively. Furthermore, an solution, followed by anaerobic achieved satisfactory results. SMCEP displayed cumulative at 801.16 mL/g TS, which 38.79% higher than that observed 22.15% solid-state 25.41% liquid pretreatment. These results indicate enzyme-pretreated can significantly enhance This study represents environmental burden energy use crop residues.