Quantification of 19 Aldehydes in Human Serum by Headspace SPME/GC/High-Resolution Mass Spectrometry

• DOI: 10.1021/acs.est.8b02745
• Publication Date: 2018-08-22T19:30:54Z
• Authors: Lalith K. Silva; Grace A. Hile; Kimberly M. Capella; Michael F. Espenship; Mitchell M. Smith; Víctor R. De Jesús; Benjamin C. Blount
• Keywords: Acrolein; Crotonaldehyde; Isotope dilution; Solid-Phase Microextraction; Tobacco smoke

Sources of human aldehyde exposure include food additives, combustion organic matter (tobacco smoke), water disinfection byproducts via ozonation, and endogenous processes. Aldehydes are potentially carcinogenic mutagenic, chronic has raised concerns about potential deleterious health effects. To aid investigations exposure, we developed a novel method to measure 19 aldehydes released from Schiff base protein adducts in serum using controlled acid hydrolysis, solid-phase microextraction (SPME), gas chromatography (GC), high-resolution mass spectrometry (HRMS). through quantified trace amounts (μg/L) stable isotope dilution. Detection limits range 0.1 50 μg/L, with calibration curves spanning 3 orders magnitude. The analysis fortified quality control material over three-month period showed excellent precision long-term stability (3–22% CV) for samples stored at −70 °C. intraday is also (CV, 1–10%). accuracy ranges 89 108% all measured aldehydes, except acrolein crotonaldehyde, two present tobacco smoke; their by this not considered robust due part reactivity vivo. However, results strongly suggest that propanal, butanal, isobutanal, isopentanal levels smokers higher than nonsmokers, thus may be useful as biomarkers smoke exposure. This will facilitate large epidemiological studies involving biomonitoring examine nonoccupational environmental exposures.