Antagonistic Estrogenic Effects Displayed by Bisphenol AF and Perfluorooctanoic Acid on Zebrafish (Danio rerio) at an Early Developmental Stage

• DOI: 10.1021/acs.estlett.8b00559
• Publication Date: 2018-10-30T21:24:15Z
• Authors: Pengyu Chen; Qiang Wang; Meng Chen; Jing Yang; Ruihan Wang; Wenjue Zhong; Lingyan Zhu; Lihua Yang
• Keywords: 01 natural sciences; 0105 earth and related environmental sciences

Bisphenol AF (BPAF) and perfluorooctanoic acid (PFOA) may display combined estrogenic effects on aquatic organisms, because BPAF and PFOA are widely present in the aquatic environment and reported to have estrogenic effects on aquatic organisms. In this study, zebrafish embryos were exposed to BPAF, PFOA, and binary mixtures of both for 7 days using estradiol (E2) and vitellogenin (VTG) as biomarkers. The estrogen receptor α (erα) expression and VTG level increased upon single exposures to BPAF (0.0372 and 0.372 μM) and PFOA (1.21 and 12.1 μM). Although both of them stimulated the P450 aromatase level, they displayed opposite effects on sex hormone-binding globulin. As a result, BPAF stimulated the E2 level but PFOA depressed it, and an antagonist effect on the E2 level was observed in the binary mixtures. The significant correlation between VTG level and erα expression suggested erα played a vital role in VTG synthesis. BPAF and PFOA could compete for the key common residue, Arg362, in the binding pocket...