Melting Properties of Peptides and Their Solubility in Water. Part 2: Di- and Tripeptides Based on Glycine, Alanine, Leucine, Proline, and Serine

• DOI: 10.1021/acs.iecr.0c05652
• Publication Date: 2021-02-08T13:02:35Z
• Authors: Hoang Tam Do; Yeong Zen Chua; Jonas Habicht; Marcel Klinksiek; Sophia Volpert; Moritz Hallermann; Max Thome; Daniel Pabsch; Dzmitry Zaitsau; Christoph Schick; Christoph Held
• Keywords: Tripeptide; Alanine

In downstream processes for peptides, crystallization is still used as the state-of-the-art separation step which knowledge about solubility of each single compound mandatory. Since determination experimental temperature-dependent data time-consuming and expensive, modeling based on physical properties such melting highly desired. Unfortunately, direct biomolecules using conventional differential scanning calorimetry not possible due to decomposition peptides before their melting. this work, fast (FSC) with heating rates up 20,000 K s–1 was applied measure 22 focus isomeric dipeptides tripeptides glycine, l-alanine, l-leucine, l-proline, l-serine. The aqueous these performed photometric method (UV/Vis spectrometer) gravimetric supersaturated solutions. Additionally, pH value crystal structure were determined in order ensure neutral species solution exclude changes solid phase. FSC-measured input thermodynamic framework PC-SAFT model peptide water. pure-component parameters following a weighted joint-parameter introduced work. This approach allows determining by joining parent amino acids. binary interactions parameter between water fitted solubility-independent osmotic coefficients mixture densities modeled good agreement solubility.