FoxO1 Knockdown Promotes Fatty Acid Synthesis via Modulating SREBP1 Activities in the Dairy Goat Mammary Epithelial Cells

• DOI: 10.1021/acs.jafc.0c05237
• Publication Date: 2020-10-15T15:16:13Z
• Authors: Qiuya He; Jun Luo; Jiao Wu; Weiwei Yao; Zhuang Li; Hui Wang; Huifen Xu
• Keywords: FOXO1; Lipogenesis; Fatty acid synthesis; Lipotoxicity

FoxO1 is a crucial transcription factor involved in lipid metabolism mouse liver through repressing key regulator of lipogenesis, sterol regulatory element binding protein 1 (SREBP1). However, it remains elusive whether plays roles the regulation fatty acid during lactation dairy goats. In this study, we aim to investigate function goat mammary epithelial cells (GMECs). We found that expression significantly upregulated compared with dry period. knockdown enhanced genes related de novo synthesis (e.g., FASN, ELOVL6 and SCD1) triacylglycerol (TAG) DGAT2 GPAM). Consistently, intracellular TAG was increased reduced overexpression cells. Immunofluorescence staining revealed insulin suppresses by promoting its nuclear export. Further, inhibits insulin-induced SREBP1 promoter activities GMECs. Moreover, via LXR response (LXRE) SREBP (SRE) located promoter. Our data reveal critical regulating