Quantitative Proteome Profiling Provides Insight into the Proteins Associated with β-Glucan Accumulation in Hull-less Barley Grains

• DOI: 10.1021/acs.jafc.0c05284
• Publication Date: 2020-12-29T02:31:40Z
• Authors: Guoqiang Zhang; Guoping Zhang; Xingquan Zeng; Qijun Xu; Yulin Wang; Hongjun Yuan; Yuhong Zhang; Tashi Nyima
• Keywords: Proteome; Xyloglucan; Fold change

The hull-less barley (Qingke) is widely planted as a staple food crop in the Tibetan area, China, and grains contains high content of β-glucan (BG). mechanisms BG synthesis accumulation qingke has not been studied at protein level. This study characterized proteins associated with during seed development. proteome profiles seeds taken 20, 30, 40 days after flowering were compared using TMT-based quantitative proteomics. A total 4283 identified, 759 being differentially expressed (DEPs) throughout Comparisons expression pattern, functions, pathway enrichment tests highlight cell wall modification, carbon energy metabolism, polysaccharide post-transcriptional modifications, vesicular transport critical biological processes related to accumulation. Furthermore, induction starch synthase, branching enzyme, pectin acetyl esterases, beta-glucosidases, beta-amylases, 1,4-beta-xylan, xyloglucan, α-amylase inhibitors, glycosyltransferases underpinned synthesis. results also indicated that involved glycolytic, gluconeogenesis, glyoxylate bypass pathways provided reducing power for storage. Parallel reaction monitoring (PRM) real-time PCR (qPCR) analyses confirmed profile obtained by current an insight into