High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli

• DOI: 10.1021/acs.jafc.0c08141
• Publication Date: 2021-02-05T19:36:17Z
• Authors: Hongxuan Wu; Jinhua Yang; Peijie Shen; Qingchen Li; Weibin Wu; Xianzhang Jiang; Lina Qin; Jianzhong Huang; Xiao Cao; Feng Qi
• Keywords: Indole-3-acetic acid; Metabolic Engineering; Heterologous; Bioproduction

Indole-3-acetic acid (IAA) is a critical plant hormone that regulates cell division, development, and metabolism. IAA synthesis in plants plant-associated microorganisms cannot fulfill the requirement for large-scale agricultural production. Here, two novel biosynthesis pathways, tryptamine (TAM) indole-3-acetamide (IAM), were developed production by whole-cell catalysis de novo an engineered Escherichia coli MG1655. When 10 g/L l-tryptophan was used as substrate, MIA-6 strain containing heterologous IAM pathway had highest titer of 7.10 (1.34 × 103 mg/g DCW), which 98.4 times more than MTAI-5 TAM catalysis. De optimized improving NAD(P)H availability, resulting increased 906 mg/L obtained MGΔadhE::icd strain, 29.7% higher control. These strategies exhibit potential E. possible industrial applications.