Pterostilbene Ameliorates DSS-Induced Intestinal Epithelial Barrier Loss in Mice via Suppression of the NF-κB-Mediated MLCK-MLC Signaling Pathway

• DOI: 10.1021/acs.jafc.1c00274
• Publication Date: 2021-03-24T10:28:17Z
• Authors: Juan Wang; Hui Zhao; Ke Lv; Wei Zhao; Ning Zhang; Fan Yang; Xiang Wen; Xiaohua Jiang; Jingrui Tian; Xinjuan Liu; Chi-Tang Ho; Shiming Li
• Keywords: Paracellular transport; Intestinal epithelium; Caco-2; Barrier function; Intestinal mucosa

The integrity of the intestinal barrier is critical for homeostasis. In this study, we investigated protective effect pterostilbene (PTE) on epithelium barrier. vitro results transepithelial electrical resistance (TEER) in Caco-2 cells indicated that PTE counteracted tumor necrosis factor α (TNFα)-induced damage. vivo pretreatment markedly ameliorated dysfunction induced by dextran sulfate sodium (DSS). Notably, epithelial tight junction (TJ) molecules were restored mice exposed to DSS. mechanism study revealed prevented myosin light-chain kinase (MLCK) from driving phosphorylation MLC (p-MLC), which crucial maintaining TJ stability. Furthermore, blunted translocation NF-κB subunit p65 into nucleus downregulate MLCK expression and then safeguard TJs integrity. These findings suggest protected through NF-κB- MLCK/p-MLC signal pathway.