Improving the Production of Punicic Acid in Baker’s Yeast by Engineering Genes in Acyl Channeling Processes and Adjusting Precursor Supply

• DOI: 10.1021/acs.jafc.1c03256
• Publication Date: 2021-08-16T08:51:46Z
• Authors: Juli Wang; Yang Xu; Roman Holic; Xiaochen Yu; Stacy D. Singer; Guanqun Chen
• Keywords: Metabolic Engineering

Punicic acid (PuA) is a high-value edible conjugated fatty with strong bioactivities and has important potential applications in nutraceutical, pharmaceutical, feeding, oleochemical industries. Since the production of PuA severely limited by fact that its natural source (pomegranate seed oil) not readily available on large scale, there considerable interest understanding biosynthesis accumulation this plant-based unusual transgenic microorganisms to support rational design biotechnological approaches for via fermentation. Here, we tested effectiveness genetic engineering precursor supply model yeast strain Saccharomyces cerevisiae. The results revealed combination feeding co-expression selected genes acyl channeling processes created an effective "push–pull" approach increase content, which could prove valuable future efforts produce industrial other