Promoting Butenyl-spinosyn Production Based on Omics Research and Metabolic Network Construction in Saccharopolyspora pogona

• DOI: 10.1021/acs.jafc.2c00285
• Publication Date: 2022-03-04T18:53:37Z
• Authors: Jie Rang; Li Cao; Ling Shuai; Yang Liu; Zirong Zhu; Ziyuan Xia; Duo Jin; Yunjun Sun; Ziquan Yu; Shengbiao Hu; Qingji Xie; Liqiu Xia
• Keywords: Metabolic pathway; Gene cluster; Metabolic Engineering

Understanding the metabolism of Saccharopolyspora pogona on a global scale is essential for manipulating its metabolic capabilities to improve butenyl-spinosyn biosynthesis. Here, we combined multiomics analysis parse S. genomic information, construct network, and mine important functional genes that affect This research not only elucidated relationship between biosynthesis primary pathway but also showed low expression level continuous downregulation bus cluster competitive utilization acetyl-CoA were main reasons reduced production. Our framework identified 148 related significantly differentially expressed, confirming polyketide synthase (PKS) succinic semialdehyde dehydrogenase (GabD) play an role in regulating Combined modification these increased overall production by 6.38-fold 154.1 ± 10.98 mg/L. results provide strategy further promoting titer.