Activation Spectra of Human Bitter Taste Receptors Stimulated with Cyclolinopeptides Corresponding to Fresh and Aged Linseed Oil

• DOI: 10.1021/acs.jafc.2c00976
• Publication Date: 2022-04-02T05:11:23Z
• Authors: Tatjana Lang; Oliver Frank; Roman Lang; Thomas Hofmann; Maik Behrens
• Keywords: Methionine sulfoxide

Linseed oil is rich in unsaturated fatty acids, and its increased consumption could aid health-promoting nutrition. However, rapid oxidation of linseed concomitant development bitterness impair consumers' acceptance. Previous research revealed that cyclolinopeptides, a group cyclic peptides inherent to oil, dominantly contribute the observed bitterness. In present study, fresh stored flaxseed were analyzed for presence cyclolinopeptides using preparative high-performance liquid chromatography combined with mass spectrometry- nuclear magnetic resonance-based identification quantification. The purified compounds tested activation all 25 human bitter taste receptors which only two responded exclusively methionine-oxidized cyclolinopeptides. Of those, methionine sulfoxide-containing cyclolinopeptide-4 elicited responses at relevant concentrations. We conclude this compound main determinant oil's propose strategies reduce