Acetylation of Sesquiterpenyl Epoxy-Cyclohexenoids Regulates Fungal Growth, Stress Resistance, Endocytosis, and Pathogenicity of Nematode-Trapping Fungus Arthrobotrys oligospora via Metabolism and Transcription

• DOI: 10.1021/acs.jafc.2c01914
• Publication Date: 2022-05-12T14:13:59Z
• Authors: Yong-Hong Chen; Long-Long Zhang; Li-Jun Wang; Xu-Tong Yue; Qun-Fu Wu; Yang Jiang; Ke-Qin Zhang; Xue-Mei Niu
• Keywords: Ceramide synthase

Sesquiterpenyl epoxy-cyclohexenoids (SECs) that depend on a polyketide synthase-terpenoid synthase (PKS-TPS) pathway are widely distributed in plant pathogenic fungi. However, the biosynthesis and function of acetylated SECs still remained cryptic. Here, we identified AOL_s00215g 273 (273) was responsible for acetylation Arthrobotrys oligospora via construction Δ273, which were absent major antibacterial nonacetylated accumulated. Mutant Δ273 displayed increased trap formation, nematicidal activities but decreased fungal growth soil colonization. Glutamine, key precursor NH3 as inducer, highly accumulated, biologically active phenylpropanoids antibiotics enriched Δ273. The endocytosis autophagosomes, with most upregulated genes involved maintaining DNA transcriptional stability pathways related to coronavirus disease exosome, suggested lack might result virus infection played role diverse antagonistic ability.