miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation

• DOI: 10.1021/acs.jafc.2c02474
• Publication Date: 2022-08-12T14:06:41Z
• Authors: Sheng-Zhong Han; Kai Gao; Shuang-Yan Chang; Hak-Myong Choe; Hyo-Jin Paek; Biao-Hu Quan; Xin-Yue Liu; Liu-Hui Yang; Si-Tong Lv; Xi-Jun Yin; Lin-Hu Quan; Jin-Dan Kang
• Keywords: C2C12; Myostatin

Myostatin (MSTN) is a growth and differentiation factor that regulates proliferation of myoblasts, which in turn controls skeletal muscle growth. It may regulate myoblast by influencing miRNA expression, the present study aimed to clarify its precise mechanism action. Here, we found MSTN–/– pigs showed an overgrowth upregulated miR-455-3p level. Intervention MSTN expression using siMSTN C2C12 myoblasts also significantly increased miR-455-3p. was directly targeted 3′-untranslated region Smad2 dual-luciferase assay. qRT-PCR, Western blotting, immunofluorescence analyses indicated overexpression or silencing promoted differentiation. Furthermore, GATA3. The levels were considerably reduced following GATA3 knockdown. Consistently, knockdown enhanced caused siMSTN. Finally, illustrated has role regulation. Taken together, identified profiles miRNAs positively In addition, revealed acts through GATA3/miR-455-3p/Smad2 cascade development.