Development of Recombinase-Aided Amplification (RAA)-Exo-Probe and RAA-CRISPR/Cas12a Assays for Rapid Detection of Campylobacter jejuni in Food Samples

• DOI: 10.1021/acs.jafc.2c02581
• Publication Date: 2022-07-20T15:25:37Z
• Authors: Shuai Zhi; Jinling Shen; Xingang Li; Yuan Jiang; Junxin Xue; Taisong Fang; Jin Xu; Xuan Wang; Yuhao Cao; Danting Yang; Zhiyuan Yao; Daniel Yu
• Keywords: Campylobacteriosis

Campylobacter jejuni is the major cause of campylobacteriosis, one most common foodborne illnesses worldwide. Here, we report development RAA-exo-probe and RAA-CRIPSR/Cas12a assays for detection C. in food samples. The two were found to be highly specific sensitive, as they log more sensitive compared traditional culture method, with thresholds 9 5 copies per reaction, respectively. These successfully detected spiked chicken samples natural meat (chicken, beef, mutton, etc.) overall less dependent on expensive equipment, only requiring a fluorescent reader. Their ease use other nucleic acid amplification-based methods indicates that these could adapted rapid, routine surveillance contamination samples, particularly work done field or poorly equipped labs.