Nelumbinis Stamen Ameliorates Chronic Restraint Stress-Induced Muscle Dysfunction and Fatigue in Mice by Decreasing Serum Corticosterone Levels and Activating Sestrin2

• DOI: 10.1021/acs.jafc.2c06318
• Publication Date: 2022-12-19T05:44:42Z
• Authors: Zhi Wang; Suwei Jin; Tianji Xia; Yongguang Liu; Yunfeng Zhou; Xinmin Liu; Ruile Pan; Yonghong Liao; Mingzhu Yan; Qi Chang
• Keywords: Corticosterone; Nutraceutical; Muscle Fatigue

Nelumbo nucifera Gaertn. is an important aquatic vegetable, and its dried stamen (Nelumbinis stamen, NS) a valuable nutraceutical usually used as herbal tea. Here, we ultrahigh-performance liquid chromatography (UPLC)–quadrupole time-of-flight mass spectrometry high-performance (HPLC) to chemically profile NS quantify their main constituent flavonoids, respectively. In total, 44 components were identified, including organic acids, monoterpene glycosides, fatty acids. Experimental mice induced with fatigue by exposure chronic restraint stress (CRS) for 8 h daily 15 days then treated aqueous extract of (0.5 1 g/kg) via gavage. significantly mitigated CRS-induced skeletal muscle dysfunction in possibly lowering serum corticosterone levels restoring Sestrin2 expression the gastrocnemius regulate metabolism, preserve mitochondrial homeostasis, promote antioxidant capacity. These results demonstrate that can be or supplement controlling stress-induced fatigue.