Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

• DOI: 10.1021/acs.jafc.2c07880
• Publication Date: 2023-01-05T11:44:35Z
• Authors: Fanqiang Meng; Yunbin Lyu; Xiaoyu Chen; Fengxia Lu; Haizhen Zhao; Yingjian Lu; Mingwen Zhao; Zhaoxin Lu
• Keywords: 0301 basic medicine; 03 medical and health sciences; Glucose; Cultured Milk Products; Polysaccharides, Bacterial; Maltose; Lactobacillus plantarum

Carbon sources alter the synthesis of exopolysaccharides (EPS) in Lactiplantibacillus plantarum. Maltose increased EPS production L. plantarum 163 6.5-fold. Subsequently, production, transcriptome, and proteome were analyzed using glucose or maltose to further clarify regulatory mechanism. A cAMP receptor protein (UniProtKB: F9UNI5) has been identified control presence by binding promoter Pcps4A-J. Overexpression gene cyaA content 4.5- 2.2-fold, respectively. Furthermore, yogurt produced with 163-cyaA had a similar viscosity that commercial Greek yogurt; it 20 83.7% greater than glucose, These findings indicated potential applications functional fermented dairy products.