Reprogramming the Metabolic Network in Kluyveromyces lactis with a Transcriptional Switch for De Novo Lacto-N-biose Synthesis

• DOI: 10.1021/acs.jafc.3c01779
• Publication Date: 2023-06-01T15:27:31Z
• Authors: Fengyi Li; Wei Ma; Lulu Liu; Kangle Niu; Daming Liu; Wencheng Yin; Xudong Zhang; Lijuan Han; Xu Fang
• Keywords: Kluyveromyces lactis; Metabolic pathway; Reprogramming; Metabolic network

Lacto-N-biose (LNB) is a member of the human milk oligosaccharide (HMO) family and synthesized via an enzymatic reaction in vitro with N-acetylglucosamine (GlcNAc) cofactors. In this study, LNB was using cell factory for first time. First, three modules were constructed Kluyveromyces lactis producing from lactose GlcNAc without addition Second, de novo pathway K. adding GlcNAc. Finally, transcriptional switch introduced into to reprogram its metabolic network improving flux GlcNAc-6-P pathway. Subsequently, final yield 10.41 g/L, similar salvage yield, achieved through The engineered provides promising technology platform industrial scale production LNB.