Integrating Genomics and Metabolomics for the Targeted Discovery of New Cyclopeptides with Antifungal Activity from a Marine-Derived Fungus Beauveria felina

• DOI: 10.1021/acs.jafc.3c02415
• Publication Date: 2023-06-13T14:46:54Z
• Authors: Minghua Jiang; Senhua Chen; Xin Lu; Heng Guo; Shuihao Chen; Xinjian Yin; Hanxiang Li; Guangyi Dai; Lan Liu
• Keywords: Citrus; Antifungal Agents; Metabolomics; Beauveria; Peptides, Cyclic

Sour rot, caused by Geotrichum citri-aurantii, is a major postharvest disease in citrus and results significant economic losses. The genus Beauveria recognized as promising source of biocontrol agents for agricultural applications. Herein, we established targeted strategy integrating genomics metabolomics to accelerate the discovery new cyclopeptides from antagonistic metabolites produced marine-derived fungus felina SYSU-MS7908. As result, isolated characterized seven cyclopeptides, including six molecules, isaridins I-N (1-6). Their chemical structures conformational analysis were extensively elucidated using spectroscopic techniques (NMR, HRMS, MS'MS data), modified Mosher's Marfey's methods, single-crystal X-ray diffraction. Notably, isaridin K (3) contains peptide backbone with an N-methyl-2-aminobutyric acid residue rarely found natural cyclopeptides. Bioassays showed that compound 2 could significantly inhibit mycelial growth G. citri-aurantii destroying cell membrane. These findings provide effective searching fungal peptides potential agrochemical fungicides also pave way further exploration applications agriculture, food, medicine.