Physiological Function of RDL1 and RDL2 Subunits of the Ionotropic GABA Receptor in the Spodoptera litura with the CRISPR/Cas9 System In Vivo

• DOI: 10.1021/acs.jafc.3c02811
• Publication Date: 2023-07-25T15:48:35Z
• Authors: Zhong Qiang Jia; Su Gui Zhang; Ying Wang; Jun Heng Pan; Fei Fan Liu; En Ling Zhan; Eman Atef Fouad; Ya Li Fu; Qi Rui Pan; Chun Qing Zhao
• Keywords: Spodoptera litura

In insect ionotropic γ-aminobutyric acid receptor (iGABAR) subunits, only resistance to dieldrin (RDL) can be individually and functionally expressed in vitro. lepidopteran, two three RDL subtypes are identified; however, their physiological roles have not been distinguished vivo. this study, SlRdl1 SlRdl2 of S. litura were knocked out using CRISPR/Cas9, respectively. The mortality larval pupal duration KOSlRdl1 KOSlRdl2 increased. flight time distance increased by 43.30%-80.66% 58.96%-198.22%, respectively, KOSlRdl1. GABA-induced current was significantly decreased 53.57%-74.28% 46.91%-63.34% the ventral nerve cord, GABA titer reduced 17.65%-28.05% 19.85%-42.46% KOSlRdl2, conclusion, necessary for transmission neural signals; could regulate capability litura. Our results provided a new avenue study lepidopteran iGABARs.