miR828a-CsMYB114 Module Negatively Regulates the Biosynthesis of Theobromine in Camellia sinensis

• DOI: 10.1021/acs.jafc.3c07736
• Publication Date: 2024-02-20T13:44:06Z
• Authors: Qifang Jin; Zhong Wang; Devinder Sandhu; Lan Chen; Chenyu Shao; Siyi Xie; Fanghuizi Shang; Shuai Wen; Ting Wu; Huiying Jin; Feiyi Huang; Guizhi Liu; Jinyu Hu; Qin Su; Mengdi Huang; Qian Zhu; Biao Zhou; Lihua Zhu; Lvwen Peng; Zhonghua Liu; Jianan Huang; Na Tian; Shuoqian Liu
• Keywords: Theobromine

Theobromine is an important quality component in tea plants (Camellia sinensis), which produced from 7-methylxanthine by theobromine synthase (CsTbS), the key rate-limiting enzyme biosynthetic pathway. Our transcriptomics and widely targeted metabolomics analyses suggested that CsMYB114 acted as a potential hub gene involved regulation of biosynthesis. The inhibition expression using antisense oligonucleotides (ASO) led to 70.21% reduction level leaves plant, verified involvement Furthermore, we found was located nucleus cells showed characteristic transcription factor. dual luciferase analysis, yeast one-hybrid assay, electrophoretic mobility shift assay (EMSA) activated CsTbS, through binding CsTbS promoter. In addition, microRNA, miR828a, identified directly cleaved mRNA CsMYB114. Therefore, conclude CsMYB114, factor promotes production theobromine, inhibited miR828a cleaving